What Influenced Edgar Allan Poes Writing Style - 771 Words

What Influenced Edgar Allan Poe’s Writing Style? Edgar Allan Poe, born in 1809, is a critic, mystery and short story writer, but most importantly a poet. Edgar’s troubled life greatly influenced his works. As a young boy, Edgar loved to compose poems for his loved ones. But as he grew older, Edgar started to experience pains and tragedies in life, causing him to abuse on opium and alcohol. These were scenes behind the years of Edgar’s dark writings. When Edgar was born, his father abandoned the entire family. Then Edgar’s mother died of tuberculosis when he was only 3 years old. He was then orphaned by John Allan and Frances Keeling Valentine Allan. Edgar, as a young child, had a good stepfather-son relationship, but as Edgar†¦show more content†¦Edgar moved in Richmond with his Aunt and her daughter named Virginia, where he worked for The Messenger magazine. His magnificent writing soon gained the magazine popularity, but his drug abuse forced his boss to eventually fire him. From there he moved up north after marrying his young cousin, Virginia. In 1847, however, his wife Virginia died of tuberculosis. This death led to Poe’s excessive drinking, which would negatively affect the rest of his life, causing variousShow MoreRelatedEdgar Allan Poe Revlutionized Literature in the 19th Century665 Words   |  3 PagesEdgar Allan Poe revolutionized the literary world of the nineteenth century. Poe is a well- known author from the early 1800’s, who was part of the dark romanticism movement. While later in life he was a brilliant writer, Edgar Allan Poe faced many problems in his early life. Although Poe experienced death within his family at a young age, the hardships he encountered are thought to have influenced his dark writing style. These death inspired elements within Poe’s writing were ahead of their timeRead MoreThe Life and Works of Edgar Allan Poe Essay1487 Words   |  6 Pages Throughout the life of Edgar Allan Poe, he suffered many unfortunate events and endured several difficult situations. Some speculate that it was these experiences that helped to formulate the famous writing style of Edgar Allan Poe. His dark tales such as The Masque of the Red Death and The Tell-Tale Heart are horrific, and his poems such as Alone and The Raven show evidence that his life experiences influenced their dreariness. Poes story plots and his own life are undeniably related andR ead MoreEdgar Allan Poe Biography. By. Alyssa Marshall.1260 Words   |  6 Pages Edgar Allan Poe Biography By Alyssa Marshall Mrs. Guinn English III March 6, 2017 Abstract Edgar Allan Poe was a writer in the â€Å"Gothic Era†, many of his stories genres are horror like â€Å" The Masque of The Red Death†, mystery such as â€Å" The Fall of The House of Usher†, lost love as in â€Å" The Raven†, and obsession such as â€Å" The Pit and The Pendulum†. Edgar’s poemsRead MoreEdgar Allan Poes Impact on American Literature1379 Words   |  6 PagesEdgar Allan Poe is one of the most influential writers of the horror genre in American history. His horror stories have impacted numerous authors and their stories over the years. Various people have tried to copy his way of writing style, but they have failed to achieve the success he did. Even though Poe is no longer living, his impact on American literature can still be felt today. Edgar Allan Poe was born in Boston, Massachusetts on January 19, 1809. Even though Edgar Allan Poe did not growRead MoreAn Analysis Of Edgar Allan Poe s The Black Cat 1650 Words   |  7 PagesBrittany White Mrs. Bey English 1101 1 April 2015 A Day in the Life Edgar Allan Poe is a very talked about writer. He has a dark, mysterious, gothic writing style. Poe was a troubled man that struggled in a lot of different areas in his life. It is believed that all of Edgar Allan Poe’s short stories were connected to his tragic life events. â€Å"The Black Cat† is one of Edgar’s well-known short stories. It is about a man that endured a series of tragic events. This man was very feminine and his lackRead MoreAnalysis Of Edgar Allan Poe s The Raven And The Fall Of The House Of Usher 896 Words   |  4 PagesEdgar Allan Poe is an extremely well known American writer and is famous for his horrific and mysterious works such as, â€Å"The Raven† and â€Å"The Fall of the House of Usher.† Poe was born in 1809 in Boston, Massachusetts in an era that seems to have many dark and ominous writers and pieces of literature originating from that time period. Poe is said to have launched the interest in many of the detective type stories that we read from modern day writers. â€Å"In the early 1800s, romanticism was the dominantRead MoreThe Tortured Poet : Edgar Allan Poe1312 Words   |  6 PagesThe Tortured Poet: Edgar Allan Poe â€Å"Beauty is the sole legitimate province of the poem† Poe, Edgar Allan. The Philosophy of Composition. 1846. The name Poe often brings to mind tales of horror and mystery, but this Poe was also a writer of sophisticated poems, capable of extreme poetic beauty within a dark genre of writing. Poe never lived the happiest of lives, but his writing is extraordinary, both for its execution, and for the sheer elegance of the words which he found to write upon the pageRead MoreInfluence That Endures Ever More: Edgar Allan Poe908 Words   |  4 PagesInfluence That Endures Ever More: Edgar Allan Poe Edgar Allan Poe once said, â€Å"Words have no power to impress the mind without the exquisite horror of their reality.† Edgar Allan Poe was one of the most influential people in literary history and his words had the great power to impress the mind. More than 150 years after the death of Edgar Allan Poe, his writings are still influencing and inspiring writers today. Edgar Allan Poe was one of the a greatest literary influences of the 19th century andRead MoreEdgar Allan Poe s Writing Style1303 Words   |  6 Pagesâ€Å"Beauty is the sole legitimate province of the poem† Poe, Edgar Allan. The Philosophy of Composition. 1846. The name Poe often brings to mind tales of horror and mystery, but this Poe was also a writer of sophisticated poems, capable of extreme poetic beauty within a dark genre of writing. Poe never lived the happiest of lives, but his writing is extraordinary, both for its execution, and for the sheer elegance of the words which he found to write upon the page. Death is among one of the recurringRead MoreLife Darker Than Night By Edgar Allan Poe961 Words   |  4 PagesLife Darker than Night Great American writer and creator of short stories, Edgar Allan Poe was born in Boston on January 19, 1809. According to Charles E. May, a literary scholar, who specializes in the study of the short story, â€Å"Poe is very important in the history of American culture †¦ , he developed short fictions as a genre that was to have a major impact on American literature†¦ in nineteenth century† (May 5). Despite the huge amount short stories written and sold, Poe unsuccessfully tried

Personal Narrative Something You ll Always Forget

Logan Fox Lora Meredith English 1020 October 12, 2014 Anesthesiology: Something you’ll always forget â€Å"Count down from ten, and soon you’ll be asleep. We’ll wake you up when the operation is over,† a common phrase from any anesthesiologist. Anesthesiology is a very big field in the world of medicine, and few know about it. From pain management, to full surgical procedures, looking back at anesthesia has shown many advancements in the medical field. Over the years, anesthetics have made some vast improvements. It is essential to know what an anesthesiologist is, the history of anesthetics, and how to become one. This is in part to understand how an anesthesiologist impacts the medical field and why one may want to become one. First of all, one must know what anesthesiology and an anesthesiologist are to understand what is going to be gone over. The history of anesthetics need to be covered also, to get a better grasp of the concept. According to Stephen J. Thomas, a professional at New York’s Presbyterian Hospital, anesthesiology is the practice of me dicine dedicated to the relief of pain and total care of the surgical patient before, during and after surgery. Stemming off of that, an anesthesiologist is a doctor (MD or DO) who practices anesthesia. They specialize in perioperative care, development of an anesthetic plan, and the administration of anesthetics (Thomas). They then use these techniques and newer techniques to properly perform surgery or other proceduresShow MoreRelatedPersonal Narrative : Something You ll Always Forget1904 Words   |  8 PagesLogan Fox Lora Meredith English 1020 October 12, 2014 Anesthesiology: Something you’ll always forget â€Å"Count down from ten, and soon you’ll be asleep. We’ll wake you up when the operation is over,† a common phrase from any anesthesiologist. Anesthesiology is a very big field in the world of medicine, and few know about it. From pain management, to full surgical procedures, looking back at anesthesia has shown many advancements in the medical field. Over the years, anesthetics have made some vastRead MoreThe As An Academic Poet2249 Words   |  9 PagesAlthough Zapruder can be considered an academic poet, currently making his living as a faculty member at UC Riverside-Palm Desert, his poetry reaches out to grasp the mundane life of everyday people, distort it into something unrecognizable, and pull in even the most indifferent reader. The subjects of his poetry vary from people and objects that are encountered on a daily basis to pop culture to scientific research. Stripped of the formalities of set structures and rhymed lines, his poems are honestRead More The Irish Potato Famine and The Holocaust in Literature Essay5665 Words   |  23 Pagespoetry? 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It’s a pretty laid-back culture and at the same time it’s a very busy, (. . .), always needing to do stuff sort of a culture as well. While Bronty appeared to consider her cultural background as inclusive ofRead MoreAmerican Literature11652 Words   |  47 PagesRenaissance Postmodernism Contemporary Puritan Times period of American Literature - 1650-1750 Content: ï‚ · ï‚ · ï‚ · errand into the wilderness be a city upon a hill Christian utopia Genre/Style: ï‚ · ï‚ · ï‚ · ï‚ · ï‚ · sermons, diaries personal narratives captivity narratives jeremiads written in plain style Effect: ï‚ · ï‚ · instructive reinforces authority of the Bible and church Historical Context: ï‚ · ï‚ · a person s fate is determined by God all people are corrupt and must be saved by Christ Read MoreLangston Hughes : The American Dream And Southern Migration With The Reality Of Prejudice2931 Words   |  12 Pagesplace as one of the greatest writers in American Literature to this day. 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It flourishes mainly inRead MoreProject Managment Case Studies214937 Words   |  860 Pagesimplied warranties of merchantability or fitness for a particular purpose. No warranty may be created or extended by sales representatives orwritten sales materials. The advice and strategies contained herein may not be suitable for your situation. You should consult with a professional where appropriate. Neither the publisher nor author shall be liable for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages. For generalRead MoreFor Against by L.G. 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Biol 130 First Midterm Notes Free Essays

Unit 1 – Introduction to the Cell Robert Hooke – built the first microscope (30x magnification); viewed slices of cork called cellula (little rooms). Antoni Van Leeuwenhoek – worked with glass huge improvement in quality of lenses nearly 300x magnification became possible first to observe: * single-celled organisms â€Å"animalcules† * protists from pond water * bacteria from his mouth – â€Å"father of microbiology† * blood cells * banded pattern in muscle cells * sperm from †¦ 1830s – Compound microscope – improved magnification and resolution and allowed visualization of objects less than 1 ? . 1000-1500x magnification Beginning of Cell Theory Robert Brown (botanist) – noticed that every plant cell contained a round structure called it ‘kernel’-nucleus Matthias Schleiden (another botanist) – all plant tissues are composed of cells; embryonic plant always arose from a single cell Theodor Schwann (zoologist) – similar observations in animal cells; recognition of structural similarities btw plants and animals! * Cell Theory formulated by Schwann Cell Theory 1. We will write a custom essay sample on Biol 130 First Midterm Notes or any similar topic only for you Order Now all organisms consist of one or more cells 2. he cell is the basic unit of structure for all organisms 3. added 20 years later: all cells arise only from pre-existing cells fact (scientific) – an attempt to state our best current understanding, based on observations and experiments(valid only until revised or replaced) Steps in Scientific Method 1. make observations 2. use inductive reasoning to develop tentative explanation (hypothesis) 3. make predictions based on your hypothesis 4. make further observations or design and carry out controlled experiments to test your hypothesis 5. nterpret your results to see if they support your hypothesis Theory – a hypothesis that has been tested critically under many different conditions andby many different investigators . using a variety of different approaches. By the time an explanation is regarded as a theory it is widely accepted by most scientists in the cell * the â€Å"solid ground† of science: evolution, germ the ory, cell theory *If a theory is thoroughly tested and confirmed over many years by such large numbers of investigators that there is no doubt of its validity †¦ it may eventually be regarded as a law. Gravity, laws of thermodynamics, laws that govern behaviour of gases ‘Strands’ of Cell Biology 13 cytology 1600s Hooke looks at cork Leeuwenhoek looks at lots of things 1800s Brown notes nuclei bio-chemistry synthesis of urea in lab fermentation done by cells! glycolysis Krebs cycle every cell comes from a cell Schleiden Schwann formulate cell theory electron microscopy stains dyes genetics Mendel, pea plants DNA chromosomes chromosome theory 1930s DNA double helix DNA sequencing Dolly the sheep! nano-technology! genetic code Light Microscopy: Bright field – light passes through specimen, contrast is slow and specimen is hard to see Phase contrast – contrast is changed by changing light in microscope DIC – uses optical modifications to change contrast between cell and background – due to density differential Staining – stain used to visualize cell and components, only some stains can be used on living cells 14 bright field phase contrast DIC unstained (sperm cells) stained blood cells tissue – small intestine Fluorescent Microscopy – fluorescent dyes bind to protein or DNA to see where they are in cells – tracks movement Electron Microscopy(Scanning Transmission): SEM – scan surface of specimen to form image by detecting electrons from outer surface. Good surface images TEM – forms image from electrons passing through specimen therefore fine details of internal organelles 16 SEM TEM Basic Properties of Cells: * are highly complex and organized * atoms molecules macromolecules (organelles ) enclosed in plasma membrane * use the same ‘genetic program’ Central Dogma * DNA RNA protein * are capable of reproducing themselves * must first replicate genetic material acquire and use energy (â€Å"bioenergetics†) and carry out a variety of chemical reactions (â€Å"cellular metabolism†) * have many processes that are highly conserved at the molecular level * membrane structure, genetic code, ATP synthesizing enzymes, actin filaments, eukaryotic flagella, †¦ * engage in many mechanical activities * transport of materials in/out, within * assembly and disassembly of structures * motility / movement * respond to environmental signals * move away or toward stimuli * respond to hormones, growth factors, etc * are capable of self-regulationâ€Å"homeostasis† most evident when control systems break down; defects in DNA replication, DNA repair, cell cycle control Two Classes of Cells – karyon = nucleus Prokaryotic Cells: lack of nucleus, NO CYTOSKELETON(very small), membrane bound organelles. Mostly unicellular. Bacteria and Archaea. Single, circular strand of DNA(fewer proteins). Cell wall in addition to PM 1-10 uM in diameter. 2 types: 1. Eubacteria – all have cells walls except for mycoplasma(resistant to antibiotics that target cell wall synthesis). Mycoplasma(smallest) Cyanobacteria (largest and most complex). 2. Archaeabacteria – all have cell walls and are known as extermophiles, occupy broad range of habitats, halophiles=salty, acidophiles=acid, thermophiles= hot. Eukaryotic Cells: 10x larger than prokaryotic cells, membrane bound nucleus/organelles. More complex DNA due to histones/proteins. 4 groups: 1. Protists- very diverse group – mostly single cells; algae, water molds, slime molds, protozoa 2. Fungi – single cell(yeast) or multi-cellular(mushrooms) and have cell walls. Heterotrophs; depend on external source of organic compounds 3. Plant cells- multi-cellular and have cell walls. . Animals- multi-cellular, no cell walls and are heterotrophs Cytoplasm – everything between plasma membrane and nuclear membrane, includes all membrane-bound organelles (except nucleus) Cytosol – only fluid component Endomembrane system – internal membranes that are either in direct contact or connected via transfer of vesicles (sacs of membrane). including: nuclea r envelope / membrane, endoplasmic reticulum (ER), Golgi apparatus, lysosomes, vacuoles Nucleus – stores genetic information Endomembrane System – creates intracellular compartments with different functions. Endoplasmic reticulum (ER; rough, smooth), Golgi apparatus, lysosomes. Mitochondria – generate energy to power the cell Chloroplasts – capture energy from sunlight, convert to carbohydrate Cytoskeleton – regulates cell shape, movements of materials within the cell, movement of the cell itself Flow of Traffic in EMS – Rough ER: synthesis of proteins for – export (secretion) – insertion into membranes – lysosomes Golgi apparatus: collection, packaging distribution Lysosomes * cell ‘stomachs’ have enzymes that can digest †¦ * all 4 classes of biological macromolecules worn-out organelles (mitochondria replaced every 10 days) * material brought into cell by phagocytosis Phagocytosis – plasma membrane engulfs smaller molecule and then called phagosome. Lysosome takes it in and digests, small particles are releases into the cytoplasm. Autophagy – lysosome digests a damaged organelle, small particles are relea sed into cytosol. mitochondria (all eukaryotic cells) and chloroplasts (plant cells): * contain DNA that encodes some (but not all) of their own proteins * have unusual double layers of membranes Origin of Eukaryotic Cells: Endosymbiont Theory * once believed that eukaryotes evolved gradually, organelles becoming more and more complex * now accepted that early eukaryotes originated as predators * certain organelles (mitochondria, chloroplasts) evolved from smaller prokaryotes engulfed by larger cell * later chloroplasts and the ability to perform photosynthesis Symbiosis – Mutual Advantage advantage to host cell: * aerobic respiration (aerobic bacteria mitochondria) * photosynthesis (cyanobacteria chloroplasts) advantage to bacteria: * protected environment supply of carbon compounds from host cell’s other prey Evidence Supporting Endosymbiont Theory mitochondria and chloroplasts †¦ * are similar size to bacteria, reproduced by fission like bacteria * have double membranes, consistent with engulfing mechanism * have their own ribosomes, which resemble those of prokaryotes rather than eukaryotes in terms of size, composition and sensitivity to antibiotics * have their own genomes, which are organized like those of bacteria last but not least: * are genetically similar to proposed ‘parent’ bacteria rather than ukaryotic cells Cytoskeleton important in: * cell shape * cell motility * movement / position of organelles * movement of materials within cell * movement of chromosomes during mitosis Cytoplasm in a living cell is never static * cytoskeleton is constantly being taken apart and rebuilt * organelles and vesicles are racing back and forth * can cross the cell in ~ 1 second * unattached proteins moving randomly, but rapidly * can visit every corner of the cell within a few seconds * contents of cytosol are in constant thermal motion Common to all cells: * selectively permeable plasma membrane * genetic code; mechanism of transcription and translation * ATP for the transfer of energy and metabolic pathways Model Organisms 45 Unit 2a – Intro to Cellular Chemistry Most Common Elements in Living Organisms: * C H O N – make up 96% – also P and S are common too * Exist as complex macromolecules and simpler forms like water and carbon dioxide nucleus – dense core in centre, consists of protons and neutrons electrons – continually orbit the nucleus # of protons – defining feature of an element = atomic number – # protons + # neutrons = mass of an atom = mass number – by default, an atom is ‘neutral’, with # protons = # electrons – electrons influence reactivity of an atom †¦ Atomic mass = atomic number + # of neutrons (electrons are neglected because mass is so small) Isotopes – same number of protons but different number of neutrons in the same element Anion – gain electron and are negatively charged Cation – lose electron and are positively charged Outermost ‘valence’ shell influences an atom’s reactivity * electrons in outermost shell valence electrons * unpaired valance electrons determine the number of bonds an atom can make * atoms with filled valance shell = most stable, atoms that are closest to filling are most reactive * elements abundant in organisms have at least one unpaired valence electron Some Definitions: covalent bonds – two or more atoms share pairs of valence electrons * strong bonds of biological systems non-covalent bonds, including * ionic bonds * hydrogen bonds (H-bonds) * hydrophobic interactions olecule – group of atoms held together by energy in a stable association compound – molecule composed of two or more different types of atoms Types of Covalent Bonds * electrons shared ‘equally’ * non-polar covalent bond * can be single (like H2), double (O2) or even triple, depending on number of electrons shared * electrons not shared equally * polar covalen t bond * one of the atoms has a stronger pull on the electrons than the other * pull on electrons = electronegativity * water is the most abundant molecule in biological organisms * human body is ~70% water water as a solvent can dissolve more types of molecules than other molecule known * the polarity of water is key to its role in biology hydrogen bonding – electrical attraction between electronegative atom and partial positive of hydrogen hydrophobic – no affinity for water – â€Å"water fearing† hydrophilic – affinity for water – â€Å"water loving† Acid-base Reaction substance that gives up (donates) protons acid (increases [H+] in solution) substance that accepts protons base (decreases [H+] in solution) chemical reaction that involves transfer of protons acid-base reaction * most olecules act as either an acid or a base * water can be both (both gives up and accepts protons) weak acid: very few molecules dissociated (acetic aci d, water) strong acid: readily gives up protons (hydrochloric acid) when pH = pKa species is 50% ionized Carbon is the most important element in biology carbon atoms give biomolecules their shape but other atoms attached to carbons determine their reactivity * critical H, N, O containing attachments called functional groups *learn orgo functional groups for this course Macromolecules * large, organized molecules that are typically created by polymerization * biological macromolecules (biomolecules) provide the structure and carry out the activities of a cell 4 groups: * carbohydrates(polysaccharides) * lipids(fats) * proteins * nucleic acids * monomers of groups are different – chemical reactions used to make the chains are similar Overview of Macromolecules 3 Proteins – more functions than any other group of macromolecule * enzymes – catalysis; accelerate chemical reactions transport – through cell membranes, in circulation * support – cytoskeleton, fibres of cartilage, hair, nails * signalling / regulatory – hormones, membrane proteins, intracellular messengers * movement- of the cell itself – contractile proteins, flagella – within the cell – motor proteins * defense – antibodies, complement proteins Proteins are Polymers * amino acids are connected in linear polymers of a spe cific sequence * 20 genetically encoded amino acid monomers to pick from * string of amino acids (AAs) = peptide or polypeptide polypeptide folded and coiled into a specific conformation = protein * sometimes 2 or more peptide chains (subunits) combine to form mature, functional protein Amino Acid Structure AAs are ionized under physiological conditions ionization increases solubililty, facilitates interactions with each other and other solutes, increases reactivity (zwitterions) 7 non-ionized ionized R group unique to each AA oxygens tend to pull electrons away, making it easy to lose proton gains a proton Amino Acid Side Chains – R Groups: * nonpolar – hydrophobic R groups no charged or electronegative atoms to form H bonds * insoluble in water * R groups bury themselves with the peptide chain to ‘hide’ from water * polar side chains – soluble in water * uncharged – but partial charges can form H-bonds * charged – groups containing a cids or bases – highly soluble in water AA are linked together by covalent peptide bonds: carbon from carboxyl group is linked to N terminus of amino group. R groups and central C’s do not participate in the bond. Condensation Reaction – making the chain Hydrolysis – breaking the chain Polypeptide chain: side chains extend from peptide-bonded backbone * chain is flexible – can rotate at single bonds on either side of peptide bonds * so side chains are not all projecting to one side! * chains can be from 2-3 to thousands of AAs in length * backbone is directional, convention is to number AA ‘residues’ starting at N terminus this is the primary sequence Sickle Cell Anemia – disease in which red blood cells are abnormally shaped. Caused by single point mutation which results in substitution of single amino acid in one chain of hemoglobin protein Protein Structure: Primary Structure – unique sequence of amino acids Secondary Structure – Folding into elements of structure, hydrogen bonding between amino acids(R groups not involved). 2 shapes: alpha helix and beta pleated sheet(parallel and antiparallel). * learn more Tertiary Structure- interactions of elements of secondary structure forming a global fold, folded into these unique shapes by ionic bonds (electrostatic),hydrogen bonds, disulphide bridges, hydrophobic interaction, van der waals – dipole-dipole(all non-covalent except for S-S). Order of amino acids determines final shape. Maintain globular shape even if very weak. Quaternary Structure – more than one polypeptide chain put together to form the final functional protein, linked by covalent and non-covalent interactions. Protein Domain – segment of polypeptide that forms a compact, stable and independently folding structure. Often the building blocks for larger, more complex proteins. Disulfide bonds * covalent stabilization of protein structure found in secreted proteins (destined for a more hostile extracellular environment) * formed in ER (oxidizing environment) Once folded, do proteins ever unfold? changes in physical or chemical conditions (pH, salt concentration, temperature) disruption of H-bonds, ionic bonds, disulfide bridges, etc that maintain the protein’s shape protein ‘denatures’ or unfolds Possible to renature Do proteins ever fold incorrectly? any mutation that leads to a missing or incorrect amino acid can lead to incorrectly folded protein WHY 32 Possible outcomes: mutation – leads to incorrectly folded protein * protein never functions properly loss of function protein folds properly at first but unfolds under certain conditions eventually loss of function * protein misfolds AND is deposited in insoluble aggregates within cell * loss of function and disruption of other aspects of cell activity * many human diseases now known to be associated with misfolded proteins . Alzheimers, cystic fibrosis, type II diabetes, retinitis pigmentosa, Parkinsons, Creutzfeldt-Jakob, some cancers *read about catalysts and enzymes in Janelle’s notes, page 8-9 Nucleic Acids: Information Polymers * deoxy ribo nucleic acid (DNA) sequence of subunits in DNA polymer directs RNA synthesis * ribo nucleic acid (RNA) * RNA directs ordering of AAs in a peptide chain * information stored as DNA sequences enables living organisms to pass on hereditary information * also allows each cell to pass on hereditary information to the next generation of cells Monomers of Nucleic Acids: Deoxyribo nucleotides – phosphate + deoxyribose + nitrogenous base(A,C, G, or T) Ribo nucleotides – phosphate + ribose + base (A,C,G, or U) Nucleic acids are linear (unbranched) polymers of nucleotides * each nucleotide consists of three parts: * a nitrogenous base a (5-carbon) pentose sugar * a phosphate group Purines = AGPyramidines= C,T and U * Ribose + base = nucleoside * Ribose + base + phosphate = nucleotide Functions of Nucleotides * monomeric units of RNA and DNA * important signal molecules within cells * cyclic adenosine monophosphate (cAMP) * important agents in energy transfer reactions * cleave off phosphate group to release stored energy * act as coenzymes – organic non-protein molecules required for enzyme function * usually adenine-containing nucleotides combined with B vitamins 8 condensation reaction 5’ end – beginning of chain. Chains always built 5’ 3’. Look at above example phosphate group is 5’ 3’ end – where new bases can be added Polymerization rxn’s are endergonic: * making phosphodiester bonds requires energy * energy comes from addition of 2 phosphate groups. * Activated nucleotides = nucleotide triphophates The most famous phosphorylated nucleotide †¦ adenosine triphosphate = ATP 11 adenine 4’ 5’ 5 6 1 2 3 9 4 8 7 1’ 3’ 2’ O P CH2 O O O– P O O O– P O –O O– OH OH O NH2 N N N N ribose adenine + ribose (= adenosine) Secondary Structure of DNA: two strands of DNA align in ‘antiparallel’ arrangement with bases facing inwards. H-bonds form between bases. P P P P P P P P C C G G A A T T P O O O O O O O O O O O C G OH P Note: 3 H-bonds between C and G, 2 between A and T. Only space in the sugar phosphate backbone is for Pyramidine and Purine to bond together. Features of DNA Double Helix * stabilized by H-bonds between complementary bases and hydrophobic interactions between bases * entire molecule water-soluble because charged phosphates backbone face outward * major and minor grooves are significant in regulation of gene transcription Higher Order DNA Structure: DNA molecules can adopt higher order structure – Allows for compact packaging and strict regulation of gene expression RNA vs DNA like DNA: sugar-phosphate backbone covalently linked by phosphodiester bonds * 4 different bases unlike DNA: * uracil (U) instead of thymine (T) * pairing is A-U, C-G * sugar is ribose instead of deoxyribose * hydroxyl group makes ribose much more reactive * RNA is much less stable than DNA Secondary Structure of RNA: like DNA: * H-bonds form between complementary ba se pairs unlike DNA: * most of the time, this base-pairing is between bases on the same strand * leads to formation of ‘stem and loop’ structures with single-stranded regions and double-stranded antiparallel regions * H-bonding is spontaneous, stabilizes the molecule final molecule is single-stranded * Complex folds can result in some RNA having catalytic activity Carbohydrates * Group of molecules that contain carbon, hydrogen and oxygen in a 1:2:1 ratio: (CH2O)n Only monomers are in this ratio, oligomers you lose water * Monomer=monosaccharide * Dimer=disaccharide * Trimer=trisaccharide/oligosaccharide Types: 1. Monosaccharides – simple sugars 2. Oligosaccharides – small chains (oligo=few) * Attached to proteins – glycoproteins * Attached to lipids – glycolipids 3. Polysaccharides – very long sugar chains Typical Structural Features of Sugar Monomers: carbonyl group (either ketone or aldehyde) * lots of -OH groups * vary in length of carbon skeleton (C3, C5, C6, †¦) – triose, pentose, hexose * isomeric forms (glucose, fructose, galactose) * identical chemical groups arranged differently * monosaccharides often form rings in solution Isomers – same atoms, different arrangements structural isomer – identical groups but bonded to different carbons stereo (optical) isomer – identical groups bonded to same carbons but in different orientations sixteen different hexose structures possible, all with formula C6H12O6 C O H C OH OH H C OH H HO C H C O H C OH H H C OH H C OH H C OH H HO C H H C OH H structural isomer stereo- isomer H C C O HO C H H C OH H C OH H HO C H H C OH H fructose glucose galactose *arrangement of hydroxyl groups make a big difference in biological function Disaccharide – 2 sugar monomer: * glucose + fructose = sucrose(table sugar) * glucose + lactose = lactose * glucose + glucose = maltose Formation of disaccharides by condensation reactions. monomers are linked when C1 of one monosaccharide binds to a C on another – often C4 geometry of bond different depending on hether OH group of C1 is in ? or ? position which C of other sugar is involved in linkage 7 C1, ? C4 ?-glucose ?-glucose maltose, ? -1,4 glycosidic bond ?-galactose ?-glucose lactose, ? -1,4 glycosidic bond (glucose has flipped over) C1, ? C4 Polymerization to build Polysaccharides starch both are storage forms for energy starch – plants; glycogen – animals both consist of ? -glucose monome rs linked by ? -1,4 bonds both coil into a helix (due to geometry of linkages) starch is mixture of unbranched amylose and branched amylopectin glycogen is highly branched lycogen Structural Polysaccharide in Plants: Cellulose 9 polymer of ? -glucose, joined by ? -1,4 linkages each glucose is flipped relative to adjacent ones allows for H-bonding between adjacent strands extremely stable most abundant organic molecule on earth parallel strands joined by H-bonds Structural Polysaccharide in Animals: Chitin a component of cell walls of fungi, exoskeletons of arthropods (insects, crustaceans), radulas of molluscs, beaks of cephalopods second most abundant organic molecule on earth like cellulose, joined by ? 1,4 linkages but rather than glucose, monomer is N-acetylglucosamine like cellulose, also strengthened by H-bonding btw strands 10 Structural Polysaccharide in Bacteria: Peptidoglycan component of bacterial cell walls the most complex CHO so far! two different alternating monomers linked by ? -1,4 bonds chain of amino acids attached to one of the sugars – peptide bonds instead of H-bonds (stronger) Significance of how monosaccharides are linked: * ? -1-4 linkages of starch and glycogen readily hydrolyzed * ? 1-4 linkages in structural polysaccharides very resistant to enzymatic degradation For example: enzymes that digest cellulose (cellulase) produced only by certain classes of bacteria, fungi and protozoa Difference between glycosidic bonds from peptide and phosphodiester bonds: in common: * condensation reactions different: * peptide and phosphodiester bonds always occur at the same position within their monomers * each sugar monomer has several hydroxyl groups, and geometry of glycosidic bonds is highly variable Functions of Carbohydrates: Structural: * cellulose, chitin and peptidoglycan Cell-cell recognition: * membrane proteins covalently bonded to oligosaccharides Energy Storage * ? -1,4 –linkages of starch and glycogen are readily hydrolyzed to release stored energy Lipids * group of carbon-containing compounds that are largely non-polar / hydrophobic * significant proportion of a given lipid molecule is hydrocarbon * the only macromolecule that is not a polymer major groups of lipids in cells: * fats / oils – energy storage * sterols * cholesterol – membrane component * steroids – hormones * * Phospholipids * major component of biological membranes Fats (Triacylglycerols, Triglycerides) * form that fat is stores in apidose tissie * glycerol with 3 fatty acids attached * the link between glycerol and fatty acid = ester bond: condenstation rxn (liberates water) * hydrophobic * fatty acid(carboxylic acid with long hydrocarbon tail) Saturated Fatty Acid – have maximum number of hydrogen atoms on each atom; straight and flexible because of only single bonds Unsaturated Fatty Acid – contain at least 1 double bond. The double bond is rigid and creates a kink in the chain. The rest of the chain however is free to rotate about C-C bonds. Cis – H on the same side of double bond; don’t solidify easily Trans – H on the opposite side of the double bond. Hydrogenation – making a fat saturated/more solid at room temperature to improve shelf life therefore less healthy. Sterols – group of steroids based on cholesterol(important component of cell membrane) Phospholipids : * 1 glycerol, 2 fatty acids, 1 phosphate group(polar head group) * Amphipathic = hydrophilic and hydrophilic regions – their most important feature with respect to biology Micelles – sphere with hydrophobic tails ‘hiding’ in centre . Can only occur with relatively short tails Lipid Bilayer: Universal Structure for all Biological Membranes composition varies with: type of organism (prokaryote vs animal vs plant vs †¦) type of cell within organism (muscle, liver, sperm, egg, †¦) type of membrane within cell (plasma membrane, Golgi, ER) inner versus outer layer different patches or ‘domains’ within a particular membrane Fig 11-4 two closely apposed sheets of lipids, studded with proteins lipids serve as permeability barrier proteins perform most of the functions carbohydrates (sugars) attached to protein and lipids in a non-random manner *all membrane lipids are amphipathic Lipid bilayers form spontaneously: hydrophobic molecules would exclude water, clustering together to minimize energy cost of organizing water molecules * form large droplets or surface film * amphipathic molecules are subject to conflicting forces * solved by formation of bilayer * energetically most favourable stable, spontaneous * lipid bilayers are †¦ * closed – no free edges * self-sealing * important feature for cell fusion, budding, locomotion Fluid Mosaic Model * The plasma membrane is described to be fluid because of its hydrophobic integral components such as lipids and membrane proteins that move laterally or sideways throughout the membrane. That means the membrane is not solid, but more like a ‘fluid’. * phospholipids are constantly moving spinning in place; travelling laterally within ‘leaflet’ * phospholipids are occasionally ‘flipped’ to the opposite leaflet during membrane synthesis but they rarely ‘flop’ back * even proteins cruise slowly through the membrane! Membrane fluidity – how easily lipid molecules move within a membrane leaflet Alignment of phospholipid tails * tightly packed tails membrane more viscous, less fluid * freely moving tails higher fluidity What aspects of phospholipid composition influence this? length of fatty acids * from 14-24 carbons, 18-20 carbons most common * degree of saturation of fatty acids # double bonds * typically one saturated fatty acid and one with one or more double bonds Cholesterol: * under physiological conditions, cholesterol makes membrane stiffer – less fluid * cholesterol can make up to 50% of plasma mem brane lipid in some animal cells Regulation of Membrane Fluidity: – fluid state must be maintained for normal cell function strategies for maintaining membrane fluidity: * change composition of membranes * alter phospholipids desaturate fatty acids (to deal with cold) eg cold water vs warm water fish * change length of FA chains (yeast, bacteria) * adjust amounts of cholesterol (animals) these mechanisms have been demonstrated in: * pond fish dealing with dramatic day / night temp differences * cold-resistant plants * extremophile bacteria living in hot springs * winter wheat preparing for autumn ^ polyunsaturated FAs * sperm reduce their cholesterol just before fertilization †¦ Functions of Lipids: * storage of chemical energy * signal molecules * vitamins * wax coating on leaves * biological membranes How to cite Biol 130 First Midterm Notes, Essay examples